Title of article :
Ferula gummosa gum exerts cytotoxic effects against human malignant glioblastoma multiforme in vitro
Author/Authors :
Afshari ، Amir R. Department of Physiology and Pharmacology - School of Medicine - North Khorasan University of Medical Sciences , Mousavi ، Hadi Pharmacological Research Center of Medicinal Plants, Medical Toxicology Research Center - Mashhad University of Medical Sciences , Mousavi ، Golrokh Pharmacological Research Center of Medicinal Plants - Mashhad University of Medical Sciences , Daneshpour Moghadam ، Sanam Department of Biology - Islamic Azad University, Tonekabon Branch , Maghrouni ، Abolfazl Department of Medical Genetics - Faculty of Medicine - Tehran University of Medical Sciences , Javid ، Hossein Department of Medical Biochemistry - Faculty of Medicine - Mashhad University of Medical Sciences , Tayarani-Najaran ، Zahra Department of Pharmacodynamics and Toxicology - School of Pharmacy - Mashhad University of Medical Sciences , Bibak ، Bahram Department of Physiology and Pharmacology - School of Medicine - North Khorasan University of Medical Sciences , Mollazadeh ، Hamid Department of Physiology and Pharmacology - School of Medicine - North Khorasan University of Medical Sciences , Hosseini ، Azar Pharmacological Research Center of Medicinal Plants - Mashhad University of Medical Sciences
Abstract :
Background and purpose: Ferula gummosa (F. gummosa), a potent medicinal herb, has been shown to possess anticancer activities in vitro. The present examination evaluated the cytotoxic and apoptogenic impacts of F. gummosa gum on the U87 glioblastoma cells. Experimental approach: MTT assay to determine the cell viability, flow cytometry by annexin V/FITC-PI to apoptosis evaluation, reactive oxygen species (ROS) assay, and quantitative RT-PCR were performed. Findings / Results: The results revealed that F. gummosa inhibited the growth of U87 cells in a concentrationand time-dependent manner with IC50 values of 115, 82, and 52 μg/mL obtained for 24, 48, and 72 h posttreatment, respectively. It was also identified that ROS levels significantly decreased following 4, 12, and 24 h after treatment. The outcomes of flow cytometry analysis suggested that F. gummosa induced a sub-G1 peak which translated to apoptosis in a concentration-dependent manner. Further examination revealed that F. gummosa upregulated Bax/Bcl-2 ratio and p53 genes at mRNA levels. Conclusion and implications: Collectively, these findings indicate that sub-G1 apoptosis and its related genes may participate in the cytotoxicity of F. gummosa gum in U87 cells.
Keywords :
Apoptosis , Bax , Bcl , 2 , Ferula gummosa , Glioblastoma
Journal title :
Research in Pharmaceutical Sciences
Journal title :
Research in Pharmaceutical Sciences
