Hepatic CYP450 gene expression, hematological and biochemical indices in Caspian roach (Rutilus caspius) induced by Endosulfan

Endosulfan is one of the organochlorine pesticides which has been used worldwide for decades. The purpose of the present study was to investigate the effect of endosulfan on hematological and P450 gene expression in Rutilus caspius. Fish were exposed to the 10% and 20% LC50 for 21 days and were sampled on days 1, 7, 14 and 21. Results showed that there were significant differences in hematological parameters among the control and treated fish (p 0.05). The highest amount of Hb, Ht and RBC was observed in the control, while WBC was highest in the 20% endosulfan treated group on day 21. According to the results alkaline phosphatase, aspartate aminotransaminase and alanine amino transaminase levels increased during the experiment. The highest levels were observed with 20% endosulfan on day 14 which were significantly different with those in the control (p 0.05). In addition, the CYP450 gene expression had the same results. We conclude that exposure to the endosulfan (especially 20%) can enhance the innate immune system in Rutilus caspius.