Title of article :
Constituents of the ripe fruits of Nauclea latifolia Sm. (Rubiaceae) and their antileishmanial activities
Author/Authors :
Wonkam ، Argan Kelly Nkwenti Department of Chemistry, Department of Organic Chemistry - Higher Teacher Training College, Faculty of Science - University of Yaoundé 1 , Njanpa ، Cyrille Armel Ngansop Department of Biochemistry - Faculty of Science - University of Yaoundé 1 , Ateba ، Joël E.T. Department of Process Engineering - National Higher Polytechnic School - University of Douala , Fongang ، Yannick S.F. Department of Chemistry - Higher Teacher Training College - University of Maroua , Awantu ، Angelbert F. Department of Chemistry - Faculty of Science - University of Bamenda , Bankeu ، Jean J.K. Department of Chemistry - Faculty of Science - University of Bamenda , Chouna ، Jean Rodolph Department of Chemistry - Faculty of Science - University of Dschang , Boyom ، Fabrice F. Department of Biochemistry - Faculty of Science - University of Yaoundé 1 , Sewald ، Norbert Faculty of Chemistry - Bielefeld University , Lenta ، Bruno N. Department of Chemistry - Higher Teacher Training College - University of Yaoundé 1
Abstract :
The MeOH extract of the ripe fruits of Nauclea latifolia Sm. (Rubiaceae) showed potent antileishmanial activity in vitro during preliminary screening (IC50 = 2.20 µg/mL) against Leishmania donovani 1S (MHOM/SD/62/1S) promastigotes. Three of the four fractions of this extract showed moderate to good activities (7.06 ≤ IC50 ≤ 91.21 µg/mL) on the same strain. The purification of the fractions through CC yielded fifteen compounds whose structures were established based on their MS and NMR data. All the isolated compounds were assessed for their antileishmanial activity against L. donovani and for their cytotoxicity towards Raw 264.7 macrophage cells. 2,6-Dimethoxybenzoquinone (1) and hederagenin (6) showed good antileishmanial activity with IC50 values of 9.94 and 19.3 µM, respectively. Compound 1 was not selective, while compound 6 displayed a good selectivity towards raw 264.7 macrophage cells (SI 7.82). These results indicate that the extract from N. latifolia fruits could be considered as a source of leishmaniacidal agents.
Keywords :
Cytotoxicity , leishmaniasis , Nauclea latifolia , Ripe fruits , Rubiaceae
Journal title :
Trends In Phytochemical Research
Journal title :
Trends In Phytochemical Research
