Title of article :
Evaluation of Acute and Subacute Toxicity of Fumaria officinalis Alkaloids in Mice
Author/Authors :
Yahiaoui ، Sonia Laboratoire de Biochimie Appliquée - Faculté des Sciences de la Nature et de la Vie - Université de Bejaia , Khamtache-Abderrahim ، Sabiha Laboratoire de Biotechnologies des plantes et Ethnobotanique - Faculté des Sciences de la Nature et de la Vie - Université de Bejaia , Otmani ، Amar Laboratoire de Biochimie Appliquée - Faculté des Sciences de la Nature et de la Vie - Université de Bejaia , Bachir-bey ، Mostapha Laboratoire de Biochimie Appliquée - Faculté des Sciences de la Nature et de la Vie - Université de Bejaia , Kati ، Djamel-Edine Laboratoire de Biochimie Appliquée - Faculté des Sciences de la Nature et de la Vie - Université de Bejaia , Lequart-Pillon ، Michelle Unité de Recherche Biologie des plantes et Innovation BIOPI EA3900-UPJV, UFR des Sciences - Université de Picardie Jules Verne , Gontier ، Eric Unité de Recherche Biologie des plantes et Innovation BIOPI EA3900-UPJV, UFR des Sciences - Université de Picardie Jules Verne , Maiza-Benabdesselam ، Fadila Unité de Recherche Biologie des plantes et Innovation BIOPI EA3900-UPJV, UFR des Sciences - Université de Picardie Jules Verne
Abstract :
Background: Fumaria officinalis is largely used in traditional medicine due to its efficiency in the treatment and prevention of numerous diseases and its large spectrum of therapeutic effects. Its multiple beneficial properties are due to its richness in bioactive substances, particularly isoquinoline alkaloids. However, few studies have addressed the toxicity of this plant. Objectives: The present work aimed to study acute and subacute toxicity of alkaloids extracted from F. officinalis using Swiss albino mice as the in vivo model. Methods: Alkaloids from the aerial parts of F. officinalis were extracted and administered to male and female Swiss albino mice. The acute and subacute toxicities were studied by monitoring the weight and histopathological study of animal bodies and organs (e.g., liver, heart, spleen, and kidneys). Results: The results revealed that mice treated with increasing doses developed serious symptoms of toxicity (i.e., respiratory problems, tremors, coma, and paralysis leading the death) and lost weight. The LD50 was estimated at 1341.11 mg/kg permitting its classification as a low-toxic plant. The microscopic observations demonstrated disturbances in the kidney and liver, but not the heart and spleen. Conclusion: The alkaloids of the aerial parts of F. officinalis expressed severe toxicity in mice, particularly at high doses. Nevertheless, the neutral fraction of alkaloids is more indicated.
Keywords :
Fumaria officinalis , alkaloids , acute toxicity , subacute toxicity , Swiss albino mice
Journal title :
Avicenna Journal of Medical Biochemistry
Journal title :
Avicenna Journal of Medical Biochemistry