Title of article :
Alpha-Amylase Immobilization: Methods and Challenges
Author/Authors :
Mafakher ، Ladan Thalassemia and Hemoglobinopathy Research Center, Health Research Institute - Ahvaz Jundishapur University of Medical Sciences , Ahmadi ، Yasin Department of Medical Laboratory Sciences - College of Sciences - Komar University of Sciences and Technology , Khalili Fard ، Javad Razi Herbal Medicines Research Center - Lorestan University of Medical Sciences , Yazdansetad ، Sajjad Laboratory Sciences Research Center - Golestan University of Medical Sciences , Rezaei Gomari ، Sina School of Computing, Engineering and Digital Technologies - Teesside University , Elyasi Far ، Babak Department of Physiology and Pharmacology - School of Medicine - Dezful University of Medical Sciences
Abstract :
Alpha-amylase is one of the most widely used enzymes in the starch industry. However, industrial application of soluble alpha-amylase is hampered by changes in pH and temperature (adverse effects on enzyme stability) and activity loss, leading to higher costs. Immobilization of alphaamylase is an efficient strategy to reduce the enzyme losing and subsequently reduces costs in this regard. Alpha-amylases are immobilized by adsorption, entrapment, covalent attachment, and cross-linking. A barrier in alpha-amylase immobilization is the large size of its substrate, namely amylose and amylopectin. Most of these immobilization methods decrease the affinity of the enzyme for its substrate as well as the maximum rate of reaction (Vmax). This review aims to study different aspects of alpha-amylase including enzyme activity, applications, structure, starch, immobilization methods, and immobilization’s obstacles to improve alpha-amylase efficiency in the industry and also lowering the costs related to providing this enzyme.
Keywords :
Alpha , amylase , Immobilization , Km , Starch , Vmax
Journal title :
Pharmaceutical Sciences
Journal title :
Pharmaceutical Sciences
