Isolation and characterization of Kaempferol from Tapinanthus globiferus growing on Balanites aegyptiaca

Objective: Tapinanthus globiferus is a hemi-parasitic plant used in ethonomedicine to treat different ailment including fungal infections. The aim of the study was to isolate the bioactive compound from leaf of T.globiferus growing on Balanites aegyptiaca. Material and Methods: The plant material was collected, identified pulverized to powder using mortar and pestle. The powdered plant material was subjected to maceration with 90% methanol to obtained crude methanol leaf extract which was further partitioned with solvent of increasing polarity to obtained n-hexane, chloroform, ethylacetate and n-butanol fractions. Results: Chromatographic separation of ethylacetate fraction using a combination of silica gel column and sephadex LH-20 column led to the isolation of flavonoid (3,5,7- trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one). The structure of the compound obtained was established by Spectroscopic analysis (FTIR, NMR). Conclusion: Chromatographic studies of ethyl acetate fraction of T. globiferus growing on Balanites aegyptiaca, afforded a 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one.To the best of our knowledge, this is the first report of isolation of this compound from Tapinanthus globiferus growing on Balanites aegyptiaca.