Author/Authors :
Shakib ، Pegah Razi Herbal Medicines Research Center - Lorestan University of Medical Sciences , Halimi ، Shahnaz Department of Microbiology - School of Medicine - Tehran University of Medical Sciences , Rezaei ، Faranak Department of Microbiology - School of Medicine - Lorestan University of Medical Sciences , Delfani ، Somayeh Department of Microbiology - School of Medicine - Lorestan University of Medical Sciences
Abstract :
Background: Biofilm-associated protein (Bap) in Acinetobacter baumanniiis an essential factor in biofilm production and persistence in the hospital environment. Objectives: This study aimed to detect the bapgene in A. baumanniiby real-time polymerase chain reaction (PCR) from clinical specimens in Khorramabad, Iran. Methods: This cross-sectional study was performed during April 2017 - April 2018 on 43 A. baumanniistrains from clinical samples collected and identified by microbiological and biochemical tests. The expression of the bapgene was evaluated by real-time PCR. Data were analyzed with SPSS version 24. Results: Out of 43 A. baumanniistrains, 23, 8, 3, 3, 3, and 3 samples were isolated from chest sputum, wounds, urine, tissues, blood, and, respectively. According to the PCR results, all isolates had the bapgene except one. Real-time PCR showed significant differences in the expression of the bapgene between A. baumanniiisolates from diverse clinical samples. The highest expression of the bapgene was found in chest sputum and wound samples and had a significant difference with other samples (P 0.0001). Conclusions: We observed the bapgene in most strains, with the high expression of this gene in chest sputum and wound samples. Therefore, further studies are recommended to find strategies to inhibit the expression of this gene and biofilm formation, which help treat infections caused by biofilm-forming A. baumanniistrains.
