Neuroprotective Effects of Fe3O4 Nanoparticles Coated with Omega-3 as a Novel Drug for Recovery of Sciatic Nerve Injury in Rats

Background: Novel nanoparticle drug therapy could be an effective method to enhance peripheral nerve regeneration. Objectives: The current study aimed to determine the effect of Fe3O4 nanoparticles (NP) coated with omega-3 on sciatic nerve regeneration in Wistar rats. Methods: The rats left sciatic nerve was crushed with a non-serrated clamp. Animals in experimental groups were given Fe 3O 4@ω 3NPs (10 or 30 mg/kg/d) for one week. The recovery of functions was then assessed over eight weeks using a walking track analysis and a hot plate test. In addition, histomorphometry was used to assess the histomorphometric factors. Results: Results showed that Fe 3O 4@ω 3NPs may have enhanced sciatic nerve regeneration and improved sensorimotor rehabilitation in comparison to the negative controls. These therapeutic effects were significantly greater in Fe 3O 4@ω 3NPs at a dose of 30 mg/kg. Furthermore, Fe 3O 4@ω 3NPs treatment, compared to negative controls, significantly reduced gastrocnemius muscle atrophy and improved histomorphometric parameters. Conclusions: It was determined that Fe 3O 4@ω 3NPs had great potential for nerve regeneration due to the ease of their manufacturing and their desired efficacy.