Investigation of Spatial Intensity Distribution by Using an Optical Diffuser in the Colorimetric Microscopy Setup

Optical diffusers are optical elements for smoothing the spatial distribution of light through irregular light scattering. Recently, the fabrication and engineering of optical diffusers has attracted many attentions. In this research, an optical diffuser was fabricated using silicon carbide ceramic powder and by placing the diffuser in the optical path of the experimental setup of optical transmission microscope, the spatial intensity distribution was investigated. The uniform and symmetric spatial distribution was achieved after placing the diffuser in the optical path, and the intensity distribution diagrams were obtained corresponding to the Gaussian distribution. Finally, two-dimensional samples based on polydimethylsiloxane substrate and Kapton tape were fabricated using a low cost and simple soft nano-lithography technique and the colorimetry and imaging of the fabricated 2D samples were investigated using this experimental setup.