Author/Authors :
Ralta ، Arti Department of Pharmacology - Postgraduate Institute of Medical Education and Research (PGIMER) , Prakash ، Ajay Department of Pharmacology - Postgraduate Institute of Medical Education and Research (PGIMER) , Kumar_M ، Praveen Department of Pharmacology - Postgraduate Institute of Medical Education and Research (PGIMER) , Kumar ، Rohit Department of Pharmacology - Postgraduate Institute of Medical Education and Research (PGIMER) , Sarma ، Phulen Department of Pharmacology - Postgraduate Institute of Medical Education and Research (PGIMER) , Bhatia ، Alka Department of Experimental Medicine and Biotechnology - Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh , Medhi ، Bikash Department of Pharmacology - Postgraduate Institute of Medical Education and Research (PGIMER) , Chakrabarti ، Amitava Department of Pharmacology - Postgraduate Institute of Medical Education and Research (PGIMER)
Abstract :
Introduction: Cognitive deficit is one of the common comorbidity accompanying epilepsy. The present study evaluated the effect of Celastrus paniculatus seed extract on seizure severity and cognitive deficit following the pentylenetetrazole (PTZ)-induced chemical kindling model. Methods: PTZ kindling model was developed by daily administration of the sub-convulsive dose of PTZ 30 mg/kg for four weeks. After four weeks of induction, the following treatment, namely sodium valproic acid (SVA) 200 mg/kg, C. paniculatus 500 mgkg, pergolide 2 mg/kg, C. paniculatus (250 mgkg)+ Pergolide (1 mg/kg), and C. paniculatus (250 mgkg)+ SVA (100 mg/kg) were administered 30 minutes prior to PTZ (30 mg/kg) injection for a period of next 14 days. Neurobehavioral parameters, including superoxide dismutase (SOD), Catalase (CAT), glutathione (GSH), and dopamine levels were assessed and the Morris water maze test (MWM) and Grip strength test (GPS) were performed. Hematoxylin Eosin (H E) staining of hippocampal cornu ammonis (CA1), CA2, CA3, dentate gyrus (DG), and frontal cortex was performed. Results: C. paniculatus (500 mg/kg) alone and in combination ( C. paniculatus (250 mgkg)+ pergolide (1 mg/kg) and C. paniculatus (250 mgkg)+ SVA (100 mg/kg)) significantly (p 0.05) reduced the seizure score, mean latency time, and distance traveled in the MWM. However, no significant effect was seen in GPS. Biochemical analysis showed elevated antioxidant markers, namely GSH, CAT, and SOD, and also elevated dopamine levels. C. paniculatus and its combination also significantly (P 0.05) protected against neuronal loss in the hippocampus and frontal cortex evidenced by H E staining Conclusion: C. paniculatus alone and in combination with other agents may have the potential to treat epilepsy and associated cognitive deficits
Keywords :
C. paniculatus , Hippocampus , Kindling , Oxidative stress , Pentylenetetrazole
