The Application of DNA-Functionalized Gold Nanoparticles for Detection of Metabolites and Nucleic Acids in Personalized Medicine

In this article, the features of DNA-functionalized Gold Nanoparticles (GNPs), including the size-dependent color, the amount of conjugated DNA, and the fluorescence quenchers will be described. DNA and aptamer conjugated GNPs can be applied for producing the colorimetric and fluorescent biosensors to detect all types of disease markers including DNA, RNA, protein and other small molecular metabolites. The early phase of this work is performed in clean buffers and serum samples. DNAconjugated GNPs delivery into the cells is recently used for intracellular diagnosis in personalized medicine. Simultaneously, DNA- functionalized GNPs can be used to deliver the antisense DNA for gene therapy applications. With targeting both diagnosis and treatment applications, DNA- functionalized GNPs can be used as a suitable approach to reach theranostics purposes (diagnosis and treatment in a simultaneous manner).