Title of article :
The Effect of Collagen and Fibrin Hydrogels Encapsulated with Adipose Tissue Mesenchymal Stem Cell-Derived Exosomes for Treatment of Spinal Cord Injury in a Rat Model
Author/Authors :
Afsartala ، Zohreh Brain and Spinal Cord Injury Research Center, Neuroscience Institute - Tehran University of Medical Science , Hadjighassem ، Mahmoudreza Brain and Spinal Cord Injury Research Center, Neuroscience Institute - Tehran University of Medical Science , shirian ، sadegh Department of Pathology - School of Veterinary Medicine - Shahrekord University , Ebrahimi-Barough ، Somaye Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences , Gholami ، Leila Department of Periodontics - Dental Research Center, School of Dentistry - Hamadan University of Medical Sciences , Parasmanesh ، Gilda Department of Immunology - School of Medicine - Tehran University of Medical Sciences , Malekshahi ، Ziba Department of Medical Biotechnology - School of Advanced Technologies in Medical - Tehran University of Medical Sciences , Karimzadehbardeei ، Latifeh Department of Animal Biology - School of Biology, College of Science - University of Tehran , Ai ، Jafar Brain and Spinal Cord Injury Research Center, Neuroscience Institute - Tehran University of Medical Science
Abstract :
Background: Mesenchymal stem cell (MSC) derived exosomes (MSC-DE) have been demonstrated to be potential candidates for the treatment of rat spinal cord injury (SCI).Objective: The effect of AD-MSC and AD-MSC-DE encapsulated into collagen and fibrin hydrogels on the treatment of SCI in a rat animal model was investigated for introducing a new effective SCI treatment methodMaterials and Methods: The AD-MSC-DE was isolated using ultra-centrifugation at 100,000×g for 120 min and characterized by different methods. Fibrin and collagen hydrogels were synthesized and then mixed with AD-MSC-DE suspension. the characterized AD-MSC-DE were encapsulated into collagen and fibrin hydrogels. eighteen adult male Wister rats were randomly classified into 3 equal groups (n=6): the control group (SCI rat without treatment), SCI rat treated with either AD-MSC-DE encapsulated in collagen hydrogel or encapsulated in fibrin hydrogel groups. the treatment approaches were evaluated using clinical, histological, and molecular assays.Results: The AD-MSC-DE encapsulated into fibrin and collagen groups showed better clinical function than the control group. The AD-MSC-DE encapsulated into fibrin and collagen also improved SCI-induced polio and leuko-myelomalacia and leads to higher expression of NF protein than the control group. In the AD-MSC-DE encapsulated into collagen and fibrin leads to up-regulation the mean levels of NEFL (23.82 and 24.33, respectively), eNOS (24.31 and 24.53, respectively), and CK19 mRNAs (24.23 and 23.98, respectively) compared to the control group.Conclusion: The AD-MSC-DE encapsulated within ECM-based hydrogel scaffolds such as collagen and fibrin can regenerate the injured nerve in SCI rats and reduce spinal cord lesion-induced central neuropathic pain.
Keywords :
Adipose Mesenchymal Stem Cell , Derived Exosomes , collagen , Fibrin , Hydrogel , Spinal cord injury
Journal title :
Iranian Journal of Biotechnology (IJB)
Journal title :
Iranian Journal of Biotechnology (IJB)
