Cholesterol Metabolism Pathway, the Main Target of Coffee

Background and objectives: Coffee as a common drink for many people has been evaluated in the preset study due to its relationship with cancer risk or prevention, regulation of cholesterol level, and anti-oxidant properties. The dysregulated genes in liver of high-fat dieted mice which were treated with coffee were evaluated via network analysis to explore molecular mechanism of the event. Methods: Data were downloaded from gene expression omnibus (GEO) and the significant differentially expressed genes (DEGs) were analyzed via protein-protein interaction (PPI) network analysis by Cytoscape V.3.7.2. The Selected DEGs were enriched via gene ontology by ClueGO. Results of PPI network analysis and gene ontology enrichment were interpreted together to find the critical genes and pathways. Results: Hmgcr, Hmgcs1, Msmo1, Nsdhl, Lss, Fdps, Idi1, Mvd, Ppara, and Hsp90aa1 were identified as the central targeted genes while “cholesterol metabolism pathway” was introduced as the main affected pathway. Conclusion: Final analysis led to determine Hmgcr, Hmgcs1, Msmo1, Nsdhl, Lss, Fdps, Idi1, and Mvd as key dysregulated genes which are related to the most biological terms of “cholesterol metabolism pathway”.