Digital Image Analysis of Diabetic Foot Ulcer Areas Using ImageJ Software (National Institute of Health, USA) in Patients Treated with Autologous Activated Platelet-rich Plasma Injection

Background: In this cross-sectional study, we investigated three diabetic foot ulcer (DFU) patients. DFU is a serious diabetes complication affecting general health and social life. Autologous regenerative therapies, such as autologous activated platelet-rich plasma (aaPRP), have been proposed for DFU management. However, precise instruments are required to measure the wound size to evaluate wound closure after treatment. Objectives: This study aimed to evaluate the DFU healing process treated by aaPRP through digital image analysis using ImageJ software (National Institute of Health, USA). Methods: The DFU patients were administered aaPRP and followed up on days 7 and 14. The healing process was analyzed through ImageJ software. Paired t-test was used to assess the wound healing process. Results: The results showed that ImageJ was useful for wound area measurement. Granulation and sloughy tissue development can be evaluated and calculated as a part of wound healing progress. Conclusions: We conclude that ImageJ facilitates DFU analysis not only about the size but also on the healing time process through visualization of granulation and sloughy tissue formation, which can represent the healing progress.