Author/Authors :
inkaya, ahmet çağkan hacettepe university - school of medicine - department of infectious diseases, Ankara, Turkey , arıbaş, emel türk n ecmettin erbakan university - meram medical faculty - department of clinical bacteriology and infectious diseases, Konya, Turkey , erayman, ibrahim n ecmettin erbakan university - meram medical faculty - department of clinical bacteriology and infectious diseases, Konya, Turkey , kandemir, bahar n ecmettin erbakan university - meram medical faculty - department of clinical bacteriology and infectious diseases, Konya, Turkey , acar, hasan necmettin erbakan university - medical faculty - department of molecular biology and genetics, Konya, Turkey , bitirgen, mehmet n ecmettin erbakan university - meram medical faculty - department of clinical bacteriology and infectious diseases, Konya, Turkey
Abstract :
Objectives: Acute viral hepatitis B may lead to chronic hepatitis in 6% of adult population. We compared the frequency of Tumor necrosis factor alpha promotor polymorphisms in chronic hepatitis B patients and people with natural immunity against hepatitis B. Materials and Methods: Chronic hepatitis B patients and age matched control cases with natural immunity to hepatitis B virus were recruited 1:1 in this study. Tumor necrosis factor alpha -238G/A and -308G/A polymorphisms were studied with PCR-RFLP. χ2 test was performed in statistical analysis. Results: A total of 101 volunteers enrolled in two study groups. Thirty-eight men and 12 women constituted the chronic hepatitis B patient group and 40 men and 11 women recruited in natural immunity group. Frequency of -238G allele was 87.5% and 97% in chronic hepatitis B and natural immunity groups, respectively. Frequency of -308G allel was 93% and 92.1% in chronic hepatitis B and natural immunity groups, respectively. Frequencies of polymorphisms at positions -238 and -308 in the promotor of tumor necrosis factor alpha gene were not different between chronic hepatitis B and natural immunity groups. Discussion: Tumor necrosis factor alpha promoter polymorphisms at -238 and -308 positions do not effect the outcome hepatitis B infection in Turkish population. Clearance of hepatitis B virus infection is multifactorial. Thus, further studies needed to identify genetic predisposition to chronic hepatitis B infection.
Keywords :
Hepatitis B Virus , tumor necrosis factor alpha promoter polymorphism , genetic predisposition to chronic hepatitis B infection
