Relationship between Sperm Parameters and DNA Fragmentation using a Halosperm Kit

Introduction: tegrity is the most important component for transferring genetic information Sperm DNA fragmentation is considered a component of male infertility. Its assessment comprises conventional semen analysis of which emphasis is placed on its evaluation in fertility clinics. In this study, we investigate the relationship between sperm parameters of motility, morphology, concentration, and DNA fragmentation level (DFLs) in sperm. Materials and Methods: 89: nfertile men were assessed by conventional sperm analysis. DFLs were determined by Halosperm, a sperm chromatin dispersion test (SCD) which was performed in both groups of patients, the low DNA fragmentation group (LFG≤30%) and high DNA fragmentation group (HFG) 30%. Results: Sperm parameters of concentration (p 0.001), motility (p 0.001) and morphology (p 0.01) in the HFG group were significantly lower than in the LF group after sperm preparation. Logistic regression model showed morphology and motility variables were predictive of DFLs. The cut off points were 5% (morphology) and 50% (motility). Conclusion: Our results have shown a negative relationship between DFLs and sperm parameters after preparation. According to a DF value of 30% in the percent of men with normal semen parameters, the high importance of performing a DF test in the clinic setting in order to determine sperm DNA problems and the presence of sperm abnormalities in patients is recommended.