Author/Authors :
hamidouche, mohamed institut pasteur of algeria - laboratory of production and development of viral veterinary vaccines, Algiers, Algeria , belmessabih, nassira institut pasteur of algeria - laboratory of production and development of viral veterinary vaccines, Algiers, Algeria , boubguira, abderrahmane institut pasteur of algeria - laboratory of production and development of viral veterinary vaccines, Algiers, Algeria , benfenatki, amina institut pasteur of algeria - laboratory of production and development of viral veterinary vaccines, Algiers, Algeria , saada, naim institut pasteur of algeria - laboratory of production and development of viral veterinary vaccines, Algiers, Algeria , sail, amina institut pasteur of algeria - laboratory of production and development of viral veterinary vaccines, Algiers, Algeria , bacha, fatima institut pasteur of algeria - laboratory of production and development of viral veterinary vaccines, Algiers, Algeria
Abstract :
Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post- vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (TAb) respectively. The authors have demonstrated that a positive correlation exists between N.I. and TAb values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log TAb) + 1.331; this serves as a base to calculate N.I. using TAb values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively.
Keywords :
Capripoxvirus , Embryonic lamb kidney cells , RM65 strain , Vaccine effectiveness , Virus neutralisation.
