Author/Authors :
armansyah, teuku syiah kuala university - faculty of veterinary medicine - pharmacology laboratory, Banda Aceh, Indonesia , barat, edi rusmansyah putra syiah kuala university - faculty of veterinary medicine - veterinary study program, Banda Aceh, Indonesia , handini, cut vela risqa syiah kuala university - faculty of veterinary medicine - veterinary study program, Banda Aceh, Indonesia , aliza, dwinna syiah kuala university - faculty of veterinary medicine - pathology laboratory, Banda Aceh, Indonesia , sutriana, amalia syiah kuala university - faculty of veterinary medicine - pharmacology laboratory, Banda Aceh, Indonesia , hamdan, hamdan syiah kuala university - faculty of veterinary medicine - reproduction laboratory, Banda Aceh, Indonesia , panjaitan, budianto syiah kuala university - faculty of veterinary medicine - clinic laboratory, Banda Aceh, Indonesia , panjaitan, budianto , sayuti, arman syiah kuala university - faculty of veterinary medicine - clinic laboratory, Banda Aceh, Indonesia , siregar, tongku nizwan syiah kuala university - faculty of veterinary medicine - reproduction laboratory, Banda Aceh, Indonesia
Abstract :
This study aimed to determine the effect of seminal vesicle extract administration to increase the concentration and motility of spermatozoa and testosterone level of kacang goat. The experiment was arranged using a randomly completely block design (RCBD). Three kacang goats were used and each goat received three treatments namely P0 (2 mL of 0.9% NaCl), P1 (1.5 mL Capriglandin, PGF2α), and P2 (2 mL of seminal vesicle extract, EVS). Semen and blood were collected two days post-treatment to measure the concentration and motility of spermatozoa and testosterone level. Testosterone levels were measured with enzyme-linked immunosorbent assay (ELISA). Duration between treatments was one week after semen collection. The result showed that the average spermatozoa concentration (x10^6) in P0; P1; and P2 respectively were 2,763.0±395.0; 2,060.00±678.2; and 2,387.0±442.7 (P 0.05), while the spermatozoa motility score in P0; P1; and P2 respectively were 3.7±1.1, 3.4±0.5, and 3.4±0.5 (P 0.05). The mean testosterone levels in P0, P1, and P2 respectively were 10.27±5.42, 18.51±19.46, and 29.57±12.96 ng/mL (P 0.05). It can be concluded that administration of EVS did not increase concentration and motility of spermatozoa but increased the level of testosterone in kacang goat.
Keywords :
Kacang goat , PGF2α , Seminal vesicle extract , Spermatozoa , Testosterone.
