Asymmetric Distribution of Artificial Boundary Lipid as Analysed by NMR Deconvolution

Asymmetric distribution of an artificial boundary lipid between the outer and inner leaflets of multilamellar vesicles (MLVs) was determined by the modified 1H-NMR peak deconvolution technique. The asymmetry was affected by the vesicle preparation method, suggesting that a relatively high energy state of lipid bilayer is required for the boundary lipid to promote its specific function.