Title of article :
Comparison of Antifungal Properties of Gold, Silver, and Selenium NanoparticlesAgainst Amphotericin B-Resistant Candida glabrata Clinical Isolates
Author/Authors :
lotfali, ensieh shahid beheshti university of medical sciences - faculty of medicine - department of medical parasitology and mycology, tehran, iran , toreyhi, hossein shahid beheshti university of medical sciences - student research committee, faculty of medicine, tehran, iran , makhdoomi sharabiani, kamyab shahid beheshti university of medical sciences - student research committee, faculty of medicine, Tehran, Iran , fattahi, azam tehran university of medical sciences - center for research and training in skin diseases and leprosy, tehran, iran , soheili, amirali shahid beheshti university of medical sciences - student research committee, faculty of medicine, Tehran, Iran , ghasemi, reza shahid beheshti university of medical sciences - student research committee, faculty of medicine, tehran, iran , keymaram, mahyar iran university of medical sciences - faculty of medicine - department of medical parasitology and mycology, Tehran, Iran , rezaee, yasaman iran university of medical science - faculty of medicine - department of medical parasitology and mycology, tehran, iran , iranpanah, sayna shahid beheshti university of medical sciences - student research committee, faculty of pharmacy, Tehran, Iran
Abstract :
Background: The present study aimed to investigate the antifungal activity ofNanoparticles (NPs) against amphotericin B-resistant Candida glabrata (C. glabrata)strains.Methods: Twelve resistant (C. glabrata) strains were isolated from archived clinicalisolates. Antifungal activity was conducted according to Clinical and LaboratoryStandards Institute’s (CLSI) guidelines, document M27-A3/S4. The Scanning ElectronMicroscope (SEM) was used to observe the morphological changes of strains exposedto each nanoparticle.Results: Minimum Inhibitory Concentration (MIC) of nanoparticles of all strains was inthe concentration range of 0.125 to 0.5 μg/Ml. The synthesized Ag-NPs showed superiorantifungal activity against (C. glabrata) compared to Se-NPs and Au-NPs. Thescanning electron microscope images revealed the difference in the fungal morphologybetween the untreated and treated fungi with nanoparticles.Conclusion: The Ag-NPs, followed by Se-NPs synthesized, revealed significant antifungalactivity against resistance regardless of their antifungal-resistant mechanisms.
Keywords :
Antifungal agents , Candida glabrata , Nanoparticles , Silver
