Nanocatalytic Application of the Green Synthesized Silver Nanoparticles for Enhancement of the Enzymatic Activity of Fungal Amylase and Cellulase

The present study aims to evaluate the effect of silver nanoparticles (AgNPs) on the enzymatic activity of fungal amylase and cellulase. The AgNPs were synthesized using aqueous fresh leaf extract of Camellia sinensis of AgNPs. The synthesis of nanoparticles was initially observed by a visible colour change and further confirmed by UV-Vis spectrum analysis. Fourier transform infrared spectroscopy (FTIR) identified the functional groups and their relevant biomolecules such as amide, alkene, carbonyl, and hydroxyl groups present in the aqueous leaf extract of C. sinensis. These biomolecules were responsible for the synthesis, capping, and stabilization of the AgNPs. The field emission scanning electron microscope (FESEM) image showed spherical and polydispersed AgNPs with a diameter of 22- 55 ±2 nm. The energy dispersive X-ray (EDX) analysis illustrates 91.19% silver in the synthesized AgNPs. The effect of synthesized AgNPs on the enzymatic activity of fungal amylase and cellulase was evaluated using the 3,5-dinitrosalicylic acid (DNSA) method. The enzymatic activity of fungal amylase and cellulase increased significantly with increased concentration of AgNPs. The enhancement in the amylase and cellulase activity achieved through nanoparticles may be further explored for its industrial applications.