Using HRM Technique to Identify Population Markers in Kurdish Cities

Background and Objective: Microsatellites are ideal markers for detecting population differences in humans and considered as potentially a useful tool and biomarkers in forensic medicine. This study aimed to examine and compare the diagnostic value of three Y-STRs loci in racial studies by HRM method. Materials and Methods: Peripheral blood samples of 200 Iranian Kurdish men living in western cities of Iran (Kermanshah, Sanandaj, Sardasht, and Ilam) were collected and analyzed for allele and haplotype frequencies using HRM technique during 2017 to 2019. Results: Most allelic replications in the AC004617 (І), AC004617 (ІІ) and AC022486 loci were related to alleles 13, 29 and 30, and 12, respectively. Also, the AC022486 locus was potentially more beneficial as a population differentiation marker than the other three studied loci. Conclusion: The HRM technique was an accurate and inexpensive method for investigating the genetic differences between the four studied populations.