Title of article :
Slow release curcumin‑containing soy protein nanoparticles as anticancer agents for osteosarcoma: synthesis and characterization
Author/Authors :
Zare‑Zardini ، Hadi Department of Biomedical Engineering - Meybod University , Soltaninejad ، Hossein Faculty of Interdisciplinary Science and Technology - Tarbiat Modares University , Ghorani‑Azam ، Adel Department of Forensic Medicine and Toxicology - School of Medicine - Urmia University of Medical Sciences , Nafisi‑Moghadam ، Reza Department of Radiology - Shahid Sadoughi University of Medical Sciences , Haddadzadegan ، Navid Department of Anesthesiology and Critical Care - Shahid Sadoughi University of Medical Sciences , Ansari ، Mojtaba Hematology and Oncology Research Center - Shahid Sadoughi University of Medical Sciences , Saeed‑Banadaki ، Houssein Department of Orthopedia - Shahid Sadoughi University of Medical Sciences , Sobhan ، Mohammad Reza Department of Orthopedia - Shahid Sadoughi University of Medical Sciences , Mozafari ، Sima Hematology and Oncology Research Center - Shahid Sadoughi University of Medical Sciences , Zahedi ، Mahlagha Department of Pathology - Shahid Sadoughi University of Medical Sciences
Abstract :
Curcumin-containing soy protein nanoparticles (curcumin–SPNs) were synthesized by desolvation (coacervation) method and characterized by SEM, DLS, FTIR, and XRD. For anticancer evaluation, osteogenic sarcoma (SAOS2) cell lines were incubated with different concentrations of nanostructures. The dialysis method was used for assessment of drug release. Intracellular reactive oxygen species (ROS) were evaluated in IC50 dose after 24 h of exposure to free curcumin and cur- cumin–SPNs. Characterization data showed that the size of drug-free SPNs and curcumin–SPNs were 278.2 and 294.7 nm, respectively. The zeta potential of drug-free SPNs and curcumin–SPNs were − 37.1 and − 36.51 mv, respectively. There was no significant difference between the control and drug-free SPNs groups in terms of cell viability (p 0.05). The viability of cells in different concentrations of the designed curcumin–SPNs in Saos2 cell line was significantly higher than free drug (p 0.05). The release of curcumin showed that more than 50% of the drug was released in the first 2 h of incubation. After this time, the slow release of drug was continued to 62–83% of drug. IC50 values of free curcumin and curcumin–SPNs (1/10) were 156.8 and 65.9 µg/mL, respectively (a free curcumin IC50 was 2.4 times more than curcumin–SPNs). Slow-release of the curcumin causes the cell to be exposed to the anticancer drug for a longer period of time. The intracellular ROS levels significantly increased in an IC50 dose after 24 h of exposure to both free curcumin and curcumin–SPNs compared with controls (p 0.05).
Keywords :
Curcumin , Soy protein , Nanoparticle , Saos2 cell lines
Journal title :
Progress in Biomaterials
Journal title :
Progress in Biomaterials
