Author/Authors :
Ehsanimehr ، Narges Department of Horticultural Science - Islamic Azad University, Yasuj Branch , Hosseinifarahi ، Mehdi Department of Horticultural Science, Sustainable Agriculture and Food Security Research Group - Islamic Azad University, Yasuj Branch , Abdipour ، Moslem Kohgiluyeh and Boyerahmad Agricultural and Natural Resources Research and Education Center - Agricultural Research, Education and Extension Organization (AREEO) , Eshghi ، Saeid Department of Horticultural Science - College of Agriculture - Shiraz University
Abstract :
Rose is an important cut flower crop throughout the world in the floriculture industry. One of the most important problems of cut roses is their short post-harvest vase life, which imposes huge losses for cut plant production and trade in the market. This study aimed to investigate the effects of γ-aminobutyric acid (GABA) and calcium chloride (CaCl2) on vase life and the ACC synthase activity of cut rose cv. ‘Jumilia’ flowers. A factorial experiment based on a completely randomized design (CRD) with 12 treatments and 3 replications was carried out in a soilless culture greenhouse. The treatments included GABA in four concentrations (0, 20, 40, and 60 mM) and CaCl2 in three concentrations (0, 0.75, and 1.5% as foliar application). The combined application of GABA and CaCl2 significantly prolonged the vase life of the cut rose flowers compared to the control. The results showed that the highest and lowest vase life were obtained from the flowers treated with 20, 40, or 60 mM GABA and 0.75 % CaCl2 and the untreated flowers, by 19.1, 18.86, 18.74, and 13.85 days, respectively. The pre-harvest application of GABA and CaCl2 inhibited the ACC synthase activity. The highest and lowest ACC synthase activity were observed in plants treated with 40 mM GABA and 1.5 and 0.75% CaCl2 and the untreated flowers by 3.57, 3.96, and 8.18 nmol g-1 FW, respectively. The combined application of GABA and CaCl2 increased the Ca content by 100.95% compared to the control plants. The highest and lowest total chlorophyll contents in the leaves were related to the plants treated with 60 mM GABA and 0.75 and 1.5% CaCl2 and the untreated flowers by 8.4, 8.24, and 6.22 mg g-1 FW, respectively. The foliar application of GABA and CaCl2 significantly (P 0.01) increased the polyamines (PAs) contents, such as putrescine (Put), spermidine (Spd), and spermine (Spm), in the petals of the treated rose flowers. So, the use of foliar application GABA 60 mM and CaCl2 1.5% is recommended due to the 38% increase in the vase life of cut rose flower.
Keywords :
ACC synthase activity , Calcium , Chlorophyll content , Polyamines
