Title of article :
Adjustment of a Fibrosis Marker, Pro-Inflammatory Cytokines, and IgE in Asthmatic Animals
Author/Authors :
Dalouchi ، Fereshteh Department of Physiology - Physiology Research Center, School of Medicine - Iran University of Medical Sciences , Sharifi Aghdam ، Zeynab Department of Physiology - Physiology Research Center, School of Medicine - Iran University of Medical Sciences , Falak ، Raza Department of Immunology - School of Medicine - Iran University of Medical Sciences , Bakhshesh ، Morteza Khomein University of Medical Sciences , Hajidazeh ، Maryam Department of Immunology - School of Medicine - Iran University of Medical Sciences , Naseroleslami ، Maryam Department of Cellular and Molecular Biology - Faculty of Advanced Science and Technology - Islamic Azad University, Tehran Medical Sciences branch , Mehrab Mohseni ، Mahdieh Department of Cellular and Molecular Biology - Faculty of Advanced Science and Technology - Islamic Azad University, Tehran Medical Sciences branch , Azizi ، Yaser Department of Physiology - Physiology Research Center, School of Medicine - Iran University of Medical Sciences , Aboutaleb ، Nahid Department of Physiology - Physiology Research Center, School of Medicine - Iran University of Medical Sciences
Abstract :
Background: A lot of patients are suffering from asthma. For decreasing the asthma symptoms, we studied the effects of conditioned medium (CM) of human amniotic membrane mesenchymal stem cells (hAM-MSCs) as a source of anti-inflammatory cytokines on splenocyte and lung tissue of asthmatic Balb/c mice. Methods: Forty mice were categorized into four groups; ovalbumin (OVA)-induced asthma, CM-treated asthma, DMEM (Dulbecco’s Modified Eagle Medium)-treated asthma, and saline control. Each group received related treatment. The lung alpha-smooth muscle actin (α-SMA) and splenocyte inflammatory cytokines and IgE were examined through Western blot analysis. Results: Western blot showed α-SMA overexpression in the OVA and DMEM groups compared with the saline group. CM therapy could significantly reverse it compared with OVA and OVA+DMEM categories by elevating IL-10 and IFN-γ and reducing IL-4, IgE, and TGF-β . Conclusion: CM treatment could improve asthma symptoms by adjusting α-SMA in lung tissue and pro-inflammatory cytokines and IgE in splenocytes.
Keywords :
Asthma , Alpha , smooth muscle actin , Mesenchymal stem cell
Journal title :
Personalized Medicine Journal
Journal title :
Personalized Medicine Journal
