The effect of 8 weeks of quercetin supplementation and intermittent exercise on gene expression of Muc5Ac, Muc4 and polyphosphate in rats with colon cancer

Background: Expression of mucosal levels would affect the function of internal organs of the body and the digestive system, such as by creating a blockage for the progression of cancerous tumors and the failure of the target tissue, especially the large intestine.Aim: The purpose of this sudy was to investigate the effect of 8 weeks of quercetin supplementation and intermittent exercise on protein levels of intestinal Muc5Ac, Muc4 and polyphosphate in rats with colon cancer.Material and Methods: Tewenty-four rats were randomly assigned into four groups including quercetin (n=6), exercise (n=6), quercetin + exercise (n=6) and control group (n=6). Colon cancer induction was provided with the use of 1,2-dimethylhydrazine for 8 weeks and daily quercetin supplementation of 50 mg/kg body weight of mice by Gavagene method. Exercise protocol was performed 5 sessions per week with intensity of 60-70%, maximum speed of 23 m/min with 2-min rest in 8 weeks. ANOVA was used to analyze data. The level of significance was set at P 0.05.Results: It was suggested that there was a significant difference in protein levels of intestinal Muc5Ac, Muc4 and polyphosphate in all groups (P 0.05). Furthermore, it was also indicated that Muc5Ac levels was significantly higher in the quercetin+ exercise group other than pther groups (P 0.05).Conclusions: It was concluded that intermittent exercise and quercetin supplementation would increase the levels of Muc5Ac and Muc4 proteins in the large intestine of mice with colon cancer.