Title of article
Mir-148 and Mir-375 Expression in Oral Squamous Cell Carcinoma Patients with Oral Infection in Comparison with Oscc Patients without Oral Infection
Author/Authors
Bahrami ، Naghmeh Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine, Craniomaxillofacial Research Center - Tehran University of Medical Sciences , Hosseini ، Farzaneh Department of Microbiology - School of Microbiology Sciences - Islamic Azad University, North Tehran Branch , Hajmali ، Mahsa Department of Biotechnology - Faculty of Biological Sciences - Islamic Azad University, North Tehran Branch , Mohamadnia ، Abdolreza Department of Biotechnology - Chronic Respiratory Diseases Research Center, School of Advanced Technologies in Medicine, National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences , Mohajeri Tehrani ، Mona Craniomaxillofacial Research Center - Tehran University of Medical Sciences , Farhangiyan ، Masoume Craniomaxillofacial Research Center - Tehran University of Medical Sciences
From page
20
To page
25
Abstract
Introduction: Oral squamous cell carcinoma is a multifactorial disease that is the sixth most common cancer worldwide. MicroRNAs have been confirmed to play a role in oral squamous cell carcinoma, acting as either oncogenes or tumor suppressor genes. This study examined the expression level and role of microR-148 and microR-375 in oral cancer. Materials and Methods: In this study, we used 30 cancer samples with infection and 30 cancer samples without infection. To analyze the expression of microRNA 375 and microRNA 148, we used real-time PCR. First, we extracted total RNA from the samples. Then, we generated cDNA from it. Finally, the obtained cDNA was used in the real-time PCR technique. Results: In cancer patients with oral infection, there was an increase in microRNA-148 expression and a decrease in microRNA-375 compared to cancer patients without oral infection. Conclusion: The downregulation of microRNA-375 and upregulation of microRNA-148 can be utilized as diagnostic biomarkers and prognostic factors in oral cancer.
Keywords
Oral cancer , OSCC , Realtime pcr , MiR , 148 , MiR , 375
Journal title
Journal of Craniomaxillofacial Research (JCR)
Journal title
Journal of Craniomaxillofacial Research (JCR)
Record number
2759253
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