Title of article
Invasive Mucormycosis and the Importance of Molecular Diagnostics
Author/Authors
Mohamadnia ، Abdolreza Chronic Respiratory Diseases Research Center, NRITLD - Shahid Beheshti University of Medical Sciences , Abassi ، Amir Jalal Department of Oral and Maxillofacial Surgery - Craniomaxillofacial Research Center, School of Dentistry - Tehran University of Medical Sciences , Mohammadi ، Farnoush Department of Oral and Maxillofacial Surgery - Craniomaxillofacial Research Center, School of Dentistry - Tehran University of Medical Sciences , Fotook Kiaei ، Zahra Department of Pulmonary and Critical Care - Craniomaxillofacial Research Center, Shariati Hospital - Tehran University of Medical Sciences , Mohajeri Tehrani ، Mona Craniomaxillofacial Research Center - Tehran University of Medical Sciences , Lookzadeh ، Somaieh Chronic Respiratory Diseases Research Center, NRITLD - Shahid Beheshti University of Medical Sciences , Yassari ، Fatemeh Chronic Respiratory Diseases Research Center, NRITLD - Shahid Beheshti University of Medical Sciences , Shafaghi ، Shadi Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases - Shahid Beheshti University of Medical Sciences , Ahmadi ، Farnaz Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases - Shahid Beheshti University of Medical Sciences , Bahrami ، Naghmeh Department of Tissue Engineering and Applied Cell Sciences - Craniomaxillofacial Research Center, School of Advanced Technologies in Medicine - Tehran University of Medical Sciences
From page
87
To page
92
Abstract
Introduction: Mucormycosis, an infection, with a high death rate, requires understanding its symptoms, diagnosis methods and treatment options due, to its growing occurrence. The research employs a molecular method to evaluate the diagnosis of the mucormycosis. Materials and Methods: In this study, we obtained 30 samples from patients undergoing diagnosis and conducted DNA extraction. Additionally, DNA extraction was carried out on 30 tissue samples suspected of infection in paraffin blocks. Subsequently, PCR and Real-time PCR were performed using targeted primers, for mucormycosis followed by analysis of the results. Results: In the research findings among 30 liquid samples 8 tested positive, for mucor using the PCR method. 10 using the Real-time PCR method. Similarly, out of 30 tissue samples, 9 cases showed mucor presence with the PCR method and 11 cases, with the Realtime PCR method. Conclusion: In this study, real-time PCR and PCR techniques showed promising and faster results in detecting Mucor than the culture approach. The molecular methods provided results that could be of great use for scenarios.
Keywords
Mucormycosis , Diagnosis , Real , time PCR
Journal title
Journal of Craniomaxillofacial Research (JCR)
Journal title
Journal of Craniomaxillofacial Research (JCR)
Record number
2759261
Link To Document