Title of article
Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture
Author/Authors
Ebrahimzadeh ، Mohammad Hadi Department of Plant Production - Faculty of Agriculture - University of Torbat Heydarieh , Naseri ، Mahboobeh Plant Production of University of Torbate Heydarieh , Younesikelaki ، Fatemeh Soghra Department of Biology - Payame Noor University , Yusefi javan ، Iman University of Torbat heydarieh , Swamy N ، Rama Kakatiya University
From page
223
To page
236
Abstract
Silybum marianum (L.) is a medicinally important herb known for producing the liver-protecting compound silymarin, an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol (plant growth regulators in various concentrations and combinations of coconut water) for the initiation of callus and silymarin production using different types of explantes (root, hypocotyls, cotyledonary leaf) of S. marianum L. was established. The free radical scavenging activity of silymarin extracted from callus cultures of these explants was investigated. In comparison to other plant growth regulators and explants studied, root explants cultured on MS medium enriched with 5.0 M NAA + 2.5 M BAP + 10% coconut water produced the most friable callus and had the highest possible level of silymarin and free radical scavenging activity. NAA + BAP + coconut water was found to be the best hormonal combination for callus initiation and mass production. This research will aid in understanding the influence of plant growth regulators and different types of explantes on the production of valuable secondary metabolites in S. marianum L. in vitro cultures.
Keywords
Antioxidant activity , callus , coconut water , Silymarin
Journal title
International Journal of Horticultural Science and Technology
Journal title
International Journal of Horticultural Science and Technology
Record number
2759439
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