Title of article
Genetic and biological signature of Potato Virus X circulating in Kazakhstan
Author/Authors
Azhimakhan ، Moldir Saken Seifullin Kazakh Agrotechnical Research University , Beisembina ، Bibigul Saken Seifullin Kazakh Agrotechnical Research University , Kapytina ، Anastasiya Institute of Plant Biology and Biotechnology , Kerimbek ، Nazym Institute of Plant Biology and Biotechnology , Gritsenko ، Dilyara Institute of Plant Biology and Biotechnology , Lv ، Dianqiu Southwest University , Baigeng ، Hu Leling Xisen Potato Industry Group Company Ltd , Khassanov ، Vadim Saken Seifullin Kazakh Agrotechnical Research University
From page
1151
To page
1157
Abstract
This research studied ten isolates of potato virus X (PVX) isolated from potato cultivars growing in Kazakhstan (KZ), Belarus (BY) and China (CN). Additionally, the potato samples were screened for Potato virus Y (PVY), Potato virus S (PVS), and Potato leafroll virus (PLRV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR). PVX was isolated from the potato varieties ‘Tamasha’, ‘Red Scarlet’, ‘Gala variety’, ‘Krone’, ‘Juwel’, ‘Gala’, and ‘b2-7’. The identified isolates of PVX were sequenced and used to construct a phylogenetic tree, which demonstrated that KZ and BY isolates form clusters closely related to isolates from Asia and Europe, however, CN isolate formed a separate clade. KZ5 and KZ7 PVX isolates showed local symptoms of mosaic and light green speckled spots on the upper leaves of Datura stramonium in pathogenicity test.
Keywords
Potato , Potato Virus X , isolates , ELISA , PCR , Targeted sequencing , Indicator plants
Journal title
Caspian Journal of Environmental Sciences (CJES)
Journal title
Caspian Journal of Environmental Sciences (CJES)
Record number
2760286
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