Title of article
Investigating the Antifungal Effects of Spirocyclopropane Oxindoles Derivatives Against Aspergillus Species
Author/Authors
Ardebilifard ، Amirreza Student Research Committee - Babol University of Medical Sciences , Hoseinnejad ، Akbar Department of Medical Mycology - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , Jafarzade ، Jalal Infectious Diseases and Tropical Medicine Research Center, Health Research Institute - Babol University of Medical Sciences , Hajizadeh Jouybari ، Nava Student Research Committee - Babol University of Medical Sciences , Rajabnia Baboli ، Aida Student Research Committee - Babol University of Medical Sciences , Khalilpour ، Asieh Department of Environmental Health Engineering - School of Public Health - Babol University of Medical Sciences , Kermani ، Firoozeh Infectious Diseases and Tropical Medicine Research Center, Health Research Institute - Babol University of Medical Sciences , Yousefifard ، Aryan Student Research Committee - Ahvaz Jundishapur University of Medical Sciences , Taghizadeh Armaki ، Mojtaba Infectious Diseases and Tropical Medicine Research Center, Health Research Institute - Babol University of Medical Sciences
From page
103
To page
112
Abstract
Background: Given the increasing resistance of Aspergillus spp. to azoles, finding effective new compounds, such as the spirocyclopropane oxindoles (4a-4b-4c) derivatives, seems necessary. The present study aimed to evaluate the antifungal activity of spirocyclopropane oxindoles (4a-4b-4c) derivatives against Aspergillus spp. Methods: In vitro, the cytotoxicity of the synthesized compounds was evaluated against MCF-7 cancer cell lines using the MTT assay. In the next step, the antifungal susceptibility of 50 Aspergillus isolates of clinical origin to spirocyclopropane oxindoles (4a-4b-4c) derivatives and itraconazole was evaluated according to CLSI (Clinical and Laboratory Standards Institute) M38A2 guidelines. Statistical analysis was performed using SPSS software, version 20, and the significance level was considered P 0.05. Results: The results revealed that 4c exhibited the lowest toxicity to MCF-7 cells among the three synthesized compounds. However, this level of toxicity was higher than control. The present study shows a significant difference between the minimum inhibitory concentration (MIC) of 4a-4b-4c oxindole derivatives of spirocyclopropane and itraconazole against Aspergillus spp. Comparing the MIC values of 4a, 4b, and 4c oxindole spirocyclopropane derivatives with each other, 4b derivatives have lower MIC values for Aspergillus flavus and Aspergillus fumigatus isolates. In addition, 4c derivatives had the highest MIC for Aspergillus terreus. Conclusion: Although the antifungal effects of spirocyclopropane oxindoles (4a-4b-4c) on Aspergillus spp. were significantly lower than itraconazole, we hope to increase the antifungal effects of these compounds with structural changes.
Keywords
Spirocyclopropane Oxindoles derivatives , itraconazole , Aspergillus
Journal title
Research in Molecular Medicine
Journal title
Research in Molecular Medicine
Record number
2761553
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