Title of article
In silico analysis of genes and molecular pathways involved in the pathogenesis of follicular lymphoma
Author/Authors
Riyadh Abdulwahid ، Al-Hasnawi Rasool Xian Jiaotong University, Xian Jiaotong University Medical Campus , Mahdi ، Mohammed H College of pharmacy - Ahl Al Bayt University , Shateri Amiri ، Bahareh Department of Internal Medicine - School of Medicine Hazrat-e Rasool General Hospital - Iran University of Medical Sciences , Koosehlar ، Eman Department of Biotechnology - Faculty of Biological Science and Technology - University of Isfahan , Kazemi ، Niloufar Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine - Iranian Blood Transfusion Organization (IBTO) , Ghiasi ، Fatemeh Department of Anesthesiology - Instructor of critical care nursing, School of Allied Medical Sciences, Ayatollah Taleghani Hospital - Ilam University of Medical Sciences , Ghobadi ، Shaghayegh Department of internal medicine - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , Rezaeeyan ، Hadi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine - Iranian Blood Transfusion Organization (IBTO)
From page
205
To page
216
Abstract
Background: Follicular lymphoma (FL) is a common form of non-Hodgkin lymphoma, characterized by abnormal B-cell growth within the germinal center. Research has shown the role of genes and molecular pathways in the pathogenesis of FL. However, the main factor of pathogenesis has not been determined. Therefore, in this study, the genes and molecular pathways related to the pathogenesis of FL were evaluated using a systems biology approach. Materials and Methods: In this study (bioinformatics analysis), the GSE32018 database was used for data analysis. This database was extracted from Gene Expression Omnibus (GEO). The sample of this database was 36, which included normal and FL samples. For this purpose, 23 cases were FL and 13 were healthy samples. Protein-protein interaction (PPI) is performed to show the interaction between DEGs. STRING software is used for this purpose. Associations between the hub genes, transcription factors, and microRNAs were assessed using the miRTarBase and TRRUST databases. The criteria used for data analysis included log fold change greater than one and p 0.05. Results: After evaluating and analyzing the data, the results showed that 866 DEGs were identified between the control and FL samples. Of this population, 231 cases of UP regulation and 635 cases of downregulation were in FL samples compared to control samples. PPI network and hub gene analyses identified 7 hub genes, including RPL37A, MRPS7, RPS14, RPS28, RPL34, RPS20, and RPS3. According to the results, hsa-miR-191-5p has the highest interactions with hub genes among miRNAs, and KDM5A has the most interactions among TFs. Conclusion: Identifying genes and molecular pathways can be effective in designing therapeutic strategies and preventing the proliferation of FL cells, thereby increasing patients’ survival.
Keywords
Follicular Lymphoma , Molecular Genetics , Bioinformatics
Journal title
Iranian Journal of Pediatric Hematology and Oncology
Journal title
Iranian Journal of Pediatric Hematology and Oncology
Record number
2764998
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