Title of article
Molecular Determinants of Virulence and Antimicrobial Resistance among Enterococcus Species Isolated from Chickens
Author/Authors
Ugwu ، Ifeoma Department of Veterinary Pathology and Microbiology - University of Nigeria , Bridget Chukwudile ، Uzoamaka Department of Veterinary Pathology and Microbiology - University of Nigeria , Clifford Ugwu ، Chidozie Department of Animal Science and Technology - Federal University of Technology
From page
47
To page
57
Abstract
Enterococci cause meat and environmental contamination during slaughter time. In this study, virulence and antimicrobial resistance (AMR) characteristics of enterococci isolated from chickens were determined. A total of 107 cloacal swabs of chickens were inoculated onto Slanetz and Bartley agar and incubated at 37ºC for 24-48 h. Gram staining, catalase, and hemolytic tests were done. AMR was determined using the disc diffusion technique against twelve antimicrobials. Molecular detection of AMR genes: blaZ, aphA, aacA-aphD, ermB, tetL, tetM, and vanC, and virulence factors: agrBEfs, efaAEfs, esp, gelE, and hyl were done on selected isolates using PCR. Ninety-five isolates were Enterococcus species. The isolates showed resistance to tetracycline, cefoxitin, amoxicillin, and imipenem and possessed tetL, tetM, ermB, aphA, vanC, aaca-aphD resistance and gelE, agrBef, efaAfs, espfs and hyl virulence genes. This is the first detection of AMR and virulence genes in multi-drug resistant enterococci among chickens in the locality. These enterococci could constitute a reservoir of virulence and resistance properties which are of animal and public health concern.
Keywords
Chicken , Virulence gene , Enterococcus species , Multi , drug resistance , Antimicrobial resistance gene
Journal title
Poultry Science Journal(PSJ)
Journal title
Poultry Science Journal(PSJ)
Record number
2765801
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