in vitro Activity of Seven Antifungals against Different Clinical Candida Species

Aims: This study aimed to determine the antifungal susceptibility profile of Candida spp. with different sources. Methods: This descriptive study was conducted in the Department of Medical Mycology of Yasuj, Iran, from 2018 to 2019. Seventy-six Candida isolates recovered from different samples were used for antifungal profiles. Candida spp. isolates were identified at the species level based on molecular methods. The antifungal susceptibility tests with fluconazole, voriconazole, itraconazole, clotrimazole, amphotericin B, nystatin, and caspofungin were assessed according to the Clinical and Laboratory Standards Institute broth microdilution method. Findings: Our study showed that all isolates species were sensitive to antifungal drugs except for 3 (3.9%) isolates resistant to fluconazole. Among the In vitro activity of triazoles against all isolates tested, voriconazole had the lowest minimum inhibitory concentration ranges of 0.5-0.015µg/ml. According to the minimum inhibitory concentration of 50%, amphotericin B (0.25μg/ml) and nystatin (0.0625μg/ml) was the most active polyenes against other Candida species. Conclusion: Generally, the clinical correlation between the minimum inhibitory concentration value of different antifungals and different Candida spp. isolates are necessary because the resistance profile of Candida spp. is varied and dependent on the different variables.