Dodonaea viscosa; Enzyme Inhibitory Potential Towards Obesity/Diabetes Management and UHPLC-QTOF-MS/MS Based Metabolite Profiling

Obesity and diabetes mellitus are generalized under Diabesity, a concerning epidemic condition that affects mankind diversely. The medicinal flora has been used as a natural remedy for controlling metabolic disorders like diabesity. Dodonaea viscosat (from the Potohar region of Pakistan) has been evaluated as a potential source for the treatment of obesity/diabetes. Different solvent compositions (DVL@100%PEE, DVL@80%HEE, DVL@60%HEE, DVL@40%HEE, DVL@20%HEE DVL@PAE) were investigated for anti-oxidant, anti-obesity and antidiabetic activities. Among all compositions, DVL@60% HEE ethanolic leaves extract showed highest TFC (89 ± 1.94 mgRE/g DE), TPC (144 ± 1.37 mgGAE/g DE), DPPH scavenging potential (IC50 = 33.75 ± 0.86 μg/mL), TAP (131.59 ±1.30 mg/g AAE), pancreatic lipase inhibition (70.82 ± 0.94%) and α-glycosidase inhibition (IC50 = 43.52 ± 0.80 µg/mL). Numerous active metabolites have been identified by the highly sensitive UHPLC-QTOF-MS/MS analytical technique including Shikimic acid, Cryptomerin B, Menisdaurin, 4-O-Caffeoylquinic acid, Chlorogenic acid, cis-Caffeic acid, Quinic acid, 4-Hydroxymethyl-2-furfural, Epicatechin, Cnidimol F, 3-Hydroxynaringenin, Asaricin, cis-Anethole, E-p-Coumatic acid, Methyl 3-O-feruloylquinate, Lappaol D, Isolariciresinol, and Urolignoside, etc that might be responsible for the substantial anti-obesity/antidiabetic attributes of D. Viscosa leaves extracts.