Title of article :
Establishment and Application of a Model for SARS-CoV-2 Infection of Human Nasal Mucosa Organoid
Author/Authors :
Yu ، Yan Nanfang Hospital - Southern Medical University , Chen ، Zexin Guangdong Research Center of Organoid Engineering and Technology , Cao ، Junyuan Wuhan Institute of Virology - Chinese Academy of Sciences , Zhou ، Minmin Wuhan Institute of Virology - Chinese Academy of Sciences , Wei ، Jinyan Nanfang Hospital - Southern Medical University , Zheng ، Hairui Nanfang Hospital - Southern Medical University , Li ، Junlang Guangzhou No.3 High School , Wang ، Wei Wuhan Institute of Virology - Chinese Academy of Sciences , Li ، Gang Nanfang Hospital - Southern Medical University
Abstract :
Background: The COVID-19 pandemic underscores the need for effective models to study SARS-CoV-2 infection and evaluate antiviral therapies. Objectives: This study aimed to develop a human nasal organoid model to assess susceptibility to SARS-CoV-2 and its variants and to evaluate the antiviral effects of compounds such as camostat, remdesivir, and bergamottin. Methods: Nasal organoids were infected with SARS-CoV-2 and HCoV-OC43 pseudoviruses, followed by wild-type and variant strains in a BSL-3 laboratory. Viral content was measured at 2, 24, and 48 hours post-infection using qPCR, and infected cells were identified via immunofluorescence. Results: The organoids demonstrated susceptibility to SARS-CoV-2 and HCoV-OC43 pseudoviruses (P 0.001) and were effectively infected with SARS-CoV-2 variants. Remdesivir, hesperidin, and bergamottin exhibited dose-dependent antiviral effects (P 0.0001). Conclusions: Human nasal organoids represent a valuable model for studying SARS-CoV-2 infection and evaluating antiviral drugs, particularly for nasal applications in vitro.
Keywords :
Organoid , Nasal Mucosa , Virus Infection , SARS , CoV , 2
Journal title :
Jundishapur Journal of Microbiology (JJM)
Journal title :
Jundishapur Journal of Microbiology (JJM)
