A New Green Method for Synthesis of Manganese Oxide Nanoparticles as Antibacterial Agent Against Oral Biofilm

One of the most common human diseases is tooth decay, which is caused by several factors. Members of the group Streptococcus mutans have been identified as the leading causes of plaque formation and tooth decay. This examine aimed to optimize the inexperienced synthesis of manganese oxide nanoparticles through Bacillus sp. in opposition to Streptococcus mutans biofilm. For this purpose, nine experiments had been designed the usage of the Taguchi method. Meanwhile, the consequences of 3 factors, manganese acetate, glucose, and incubation time, had been investigated at 3 levels. The effects confirmed that the nanoparticles synthesized in experimental situations nine containing 1 mg/ml manganese acetate, eight mg/ml glucose, and seventy two h of incubation time had the best antibacterial interest in opposition to Streptococcus mutans. The characterization of synthesized nanoparticles become completed through UV-Visible spectroscopy, Fourier transforms infrared (FTIR) spectroscopy, X-ray diffraction (XRD), subject emission scanning electron microscopy (FESEM), X-ray power diffraction spectroscopy (EDX), and transmission electron microscopy (TEM). The effects acquired from the evaluation of checks performed, consisting of crystal shape and fuzzy identification, appearance, and length of nanoparticles, evaluation of chemical properties, showed the premiere situations for nanoparticle synthesis. The average size of nanoparticles synthesized using field scanning electron microscope image was determined to be 19 nm. This study showed that manganese oxide nanoparticles produced by the green synthesis method have optimal antibacterial effects against dental biofilms that cause tooth decay.