Title of article
Cytotoxic Effect of Biebersteinia multifida Alcoholic Extracts on MCF-7, HeLa, and A2780 Cell Lines
Author/Authors
Irvani ، Z Department of Traditional Medicine - Neuroscience Research Center, Institute of Neuropharmacology, Faculty of Traditional Medicine - Kerman University of Medical Sciences , Mehrbani ، M Herbal and Traditional Medicines Research Center - Kerman University of Medical Sciences , Farzin ، H Razi Vaccine and Serum Research Institute - Agricultural Research, Education and Extension Organization (AREEO) , Majidi ، B Razi Vaccine and Serum Research Institute - Agricultural Research, Education and Extension Organization (AREEO) , Mohammadi ، A Razi Vaccine and Serum Research Institute - Agricultural Research, Education and Extension Organization (AREEO) , Toroghi ، R Razi Vaccine and Serum Research Institute - Agricultural Research, Education and Extension Organization (AREEO) , Motevasselian ، M Herbal and Traditional Medicines Research Center - Mashhad University of Medical Sciences
From page
609
To page
619
Abstract
Conventional cancer treatments are costly and have different serious side effects for patients. Natural herbal treatments are widely accepted among people because of their minimal side effects, although there is little scientific knowledge about them. One of these remedies utilizes the root of Biebersteinia multifidi that has been used for years in Iran to treat different chronic genital diseases. The current study examined the effects of methanolic and ethanolic extracts of B. multifida (induction of necrosis and apoptosis) on breast cancer (MCF- 7), ovarian cancer (A2780), and human cervix cancer (HeLa) cell lines in comparison with normal breast cells. These effects were determined to be morphological alterations in cell light microscopy, by flow cytometry (staining with annexin V and propidium iodide), and by measuring live cells and inhibition concentrations by MTT assay. IC50 of B. multifida on the MCF-7 cell line (methanolic extract) was 400 μg/ml and for A2780 was 250 μg/ml. The IC50 amount of B. multifida on the MCF-7 cell line (ethanolic extract) was 750 μg/ml and 1500 for A2780. Results demonstrated that apoptosis and necrosis occurred in MCF-7 and A2780 following the addition of ethanolic and methanolic extracts of B. multifida to the medium. These findings confirmed the anticancer effects of mehthanolic extracts of Biebersteinia multifida root and its safety for normal cells; thus, it can be applied in cancer therapy as a novel medication.
Keywords
Biebersteinia multifida , traditional medicine , extract , MCF , 7 , HeLa , A2780 , cancer , cytotoxicity
Journal title
Archives of Razi Institute
Journal title
Archives of Razi Institute
Record number
2772078
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