Title of article
Inhibitory Effect of Hemiscorpius lepturus Scorpion Venom Fractions on Tachyzoites of Toxoplasma gondii
Author/Authors
Khaleghi Rostamkolaie ، Lida Department of Parasitology - Faculty of Veterinary Medicine - Shahid Chamran University of Ahvaz , Hamidinejat ، Hossein Department of Parasitology - Faculty of Veterinary Medicine - Shahid Chamran University of Ahvaz , Razi Jalali ، Mohammad Hossein Department of Parasitology - Faculty of Veterinary Medicine - Shahid Chamran University of Ahvaz , Najafzadeh Varzi ، Hossein Department of Pharmacology - Cellular and Molecular Biology Research Center - Babol University of Medical Sciences , Seifi Abadshapouri ، Massoud Reza Department of Parasitology - Faculty of Veterinary Medicine - Shahid Chamran University of Ahvaz , Jafari ، Hedieh Department of Venomous Animal and Biological Product - Razi Vaccines and Serum Research Institute - Agricultural Research Education and Extension Organization
From page
79
To page
89
Abstract
Background: The present study determined the effect of the fractions obtained from Hemiscorpius lepturus scorpion venom on the tachyzoite of Toxoplasma gondii. Methods: The fractions of dried venom of He. lepturus scorpion of Khuzestan Province, southern Iran in 2019 were isolated through gel filtration chromatography, and then tachyzoites were exposed to fractions of venom at different concentrations. Trypan blue counting and MTT were applied to assay tachyzoite viability, and the inhibition of the cellular growth of fractions in Vero cells was evaluated. Results: The maximum effect on tachyzoite was observed in fraction 5 of venom. To further separate the protein, fraction 5 was used in high-performance liquid chromatography assay to purify its proteins. Based on the results of HPLC of fraction 5, among which the second peak, a peptide with 10 KDa representing a more potent effect in eliminating the tachyzoite of T. gondii. Conclusion: The scorpion venom-purified fractions possess anti-parasitic activity against the tachyzoite of T. gondii and can be used in parasite-controlling studies.
Keywords
Scorpion , Hemiscorpius lepturus , Toxoplasma gondii , Tachyzoites , Therapeutic effect
Journal title
Iranian Journal of Parasitology (IJP)
Journal title
Iranian Journal of Parasitology (IJP)
Record number
2773070
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