Human T cell derived exosome, natural nano-particles, elicits anti-tumor effect on human solid tumor cells in vitro

Aims: To evaluate the anti-tumor effect of the T cell-exosome on breast cancer MCF-7, lung cancer A549 and liver carcinoma HepG2 cells. Methods: Human T cell derived exosomes were isolated from T cell using ultracentrifugation. The expression of the CD9 and CD81 in T cell and T cell-derived exosome, and exosome morphology was assessed using western blotting and TEM image, respectively. The anti-cancer effect of the exosome on cancer cell proliferation was measured using MTT assay. Also, the apoptotic cell percentages in treated cells were assessed by Annexin/PI staining and flowcytometry. Results: According to results, exosome therapy resulted in a reduction in the viability of the MCF-7, lung cancer A549 and liver carcinoma HepG2 cells within 12, 24, 48 and 72 hours of treatment. As well, exposure with exosomes resulted in an improvement in the apoptosis of the all cell lines within 48 hours of treatment. Conclusion: Concerning the results, T cell derived exosome could be an effective plan for treating human solid tumors.