Crosstalk between antioxidant enzymes and interleukin-10 in rats fed a high-fat diet: The impact of exercise and lctobacillus bifidus supplementation

The present study will investigate the changes and correlation between the antioxidant enzyme superoxide dismutase (SOD) and the expression of interleukin 10 (IL-10) in rats fed a high-fat diet (HFD) after 6 weeks of aerobic exercise supplemented with the probiotic Lactobacillus bifidus. 40 male Wistar rats were divided into 5 groups (8 each): healthy control, high-fat diet (HFD), HFD+aerobic exercise (Tr), HFD+probiotic supplementation (Sup), and HFD+Tr+Sup. The rats in the HFD group received a special high-fat diet for 2 months prior to the main protocol. The rats in the exercise groups ran on a rodent treadmill for 6 weeks, 5 days a week. Lactobacillus bifidus supplementation was gavaged at a dose of 1 cc to the rats in the supplementation groups after each exercise session. Finally, the rats were sacrificed, and the IL-10 variable was measured using the Real-Time PCR method. The results of the present study indicated that the high-fat diet increased cholesterol and triglyceride levels (p 0.05). The HFD and HFD+Sup groups showed a significant decrease in SOD compared to the healthy control group (p 0.05). All treatment groups demonstrated a significant increase in SOD compared to the HFD group (p 0.05). The HFD+Tr and HFD+Tr+Sup groups showed a significant increase in IL-10 compared to the HFD group (p 0.05). However, the correlation between SOD and IL-10 was not confirmed (p 0.05). There was no correlation between the antioxidant enzyme and IL-10. Nevertheless, exercise and Lactobacillus bifidus were able to regulate the lipid profile, SOD, and IL-10 after a high-fat diet.