Author/Authors :
Farrokhyar ، Sajad Department of Biology - Islamic Azad University, Tehran Science and Research Branch , Baharara ، Javad Department of Biology - Research Center for Animal Development Applied Biology - Islamic Azad University, Mashhad Branch , Eidi ، Akram Department of Biology - Islamic Azad University, Tehran Science and Research Branch , Hayati Roodbari ، Nasim Department of Biology - Islamic Azad University, Tehran Science and Research Branch
Abstract :
Objective: In recent years, in vitro maturation (IVM) has become the focus of fertility maintenance, and infertility treatment. The aim of this study is development of oocytes during folliculogenesis and oogenesis is greatly influenced by the presence of BMP-7, BMP-15, and GDF-9 genes, which are present in exosomes generated from bone marrow stem cells. Materials and Methods: In the experimental study, we investigated how exosomes obtained from bone marrow stem cells affected development and expansion of ovarian granulosa cells (GCs) in NMRI mice. In this in vitro experiment, bone marrow stem cells were isolated from mice’s bone marrow, and after identification, exosomes were recovered. Exosome doses of 100, 50, and 25 μg/ml were applied to GCs before using MTT assay to measure survival rates and quantitative reverse-transcription polymerase chain reaction (PCR) to measure expression of the BMP-7, BMP-15, and GDF-9 genes. Results: The results showed that the GCs treated with exosomes concentrations of 25, 50, and 100 μg/ml significantly increased bioavailability, growth and proliferation and it also increased expression level of BMP-7, BMP-15 and GDF-9 genes compared to the controls. Conclusion: Findings of this study indicated that exosomes derived from bone marrow stem cells improved growth of GCs in NMRI mice and they were a good candidate for further clinical studies to improve quality of the assisted reproductive techniques.
Keywords :
Annexin , Exosomes , Granulosa Cells , Stem Cells
