Author/Authors :
Aftabsavad ، Somayeh Department of Biology - Islamic Azad University, Tehran Science and Research Branch , Noormohammadi ، Zahra Department of Biology - Islamic Azad University, Tehran Science and Research Branch , Moini ، Ashraf Department of Endocrinology and Female Infertility - Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine - Academic Center for Education, Culture and Research (ACECR) , Karimipoor ، Morteza Department of Molecular Medicine - Biotechnology Research Center - Pasteur Institute of Iran
Abstract :
Objective: This study aimed to investigate the relationship between follicular fluid Bisphenol A (BPA) concentrationswith alterations in the expressions of NOTCH1-3, CASPASE 3/7, HLA-G, and ICAM-1 genes and the number ofretrieved mature oocytes (MII oocyte) in the cumulus cells of infertile poor ovarian response stimulates women. Materials and Methods:In this prospective cohort study, 80 infertile unexpected poor ovarian response (POR) subjectswere selected on the basis of subgroup 1a of the POSEIDON classification. They were divided into two groups: group1 consisted of 40 women, each with a higher number of metaphase II (MII) oocytes (G1, 3-4 oocytes retrieved), whilegroup 2 comprised of 40 women, each with a lower number of MII oocytes (G2, ≤2 oocytes retrieved). The expressionsof the studied genes were evaluated by quantitative-real time polymerase chain reaction (PCR). The concentration ofBPA in follicular fluid was measured with HPLC. Results: The expression levels of NOTCH1-3, HLA-G, and ICAM-1 genes were significantly lower in G2 than G1(P 0.05). Meanwhile, CASPASE 3/7 expression levels were higher in unexpected POR patients in G2 compared toG1 (P 0.05). There was a significant direct correlation between the levels of NOTCH1-3, HLA-G and ICAM-1 geneexpressions and there was also a significant inverse correlation (P lt;0.05) between the levels of CASPASE 3/7, with thenumber of MII oocytes and embryo development between the two groups. The concentration of BPA in the follicularfluids of G2 was higher compared to G1 (P 0.05). Conclusion: A higher concentration of BPA was associated with a lower number of mature oocytes and oocyte qualityin these patients. Also, alterations of NOTCH1-3, CASPASE 3/7, HLA-G, and ICAM-1 transcript levels in unexpectedPOR women were associated with BPA concentration.
