Molecular detection of genes responsible for multidrug resistance in uropathogenic Esherichia coli

Urinary tract infections due to urinary pathogenicity Escherichia coli (UPEC) is one of the most important diseases in the world. Escherichia coli encodes a wide range of virulence factors that are closely related to bacterial etiology. The purpose of the current study was to assess the presence of various phenotypic pathogenic markers in UPEC isolates and determine their correlation with antibiotic resistance patterns. Twenty-four uropathogenic E. coli (UPEC) isolates were collected from patients with various clinical manifestations of urinary tract infections and the isolates were identified by Vitek 2 automated system. Antibiotic susceptibility was measured using the disc diffusion method. Following biofilm, capsule, and hemolysin production, the csgA, tos A, and class 1 intgrons genes were detected, and finally the tosA gene sequence was executed. The sensitivity of bacterial isolates to ten different antibiotics was tested, and the bacteria varied in their resistance to these tested antibiotics, as all bacterial isolates were multi-resistant to antibiotics multi-drug resistance (MDR). The results of the formation of biofilms by the tube method showed a variation in terms of biofilm formation. The number of isolates that formed a strong biofilm was 8 (33.3%), which formed an average biofilm of 10 (41.7%), and finally the number of isolates that formed a weak biofilm was 6 (25%). DNA was obtained from all isolates under study and investigate the genes of virulence factors by using polymerase chain reaction. The results showed that 100% of the Escherichia coli isolates contained the gene, tos A, csg A gene and class 1 integrons.