Title of article :
Immunological effects of Penicillium digitatum in rabbits
Author/Authors :
AUDA ، Shaimaa Awadh College of Dentistry - Al-Muthanaa University , KHUDHUR ، Hasan Raheem College of Education for Pure Science - Al-Muthanna University , HASSON ، Shaimaa Obaid Department of Biotechnology - College of Biotechnology - Alqasim green University , AL-SHEMERY ، Maryam Kadhim Department of Medical Laboratory Technique - Al-Zahrawi University , JUDI ، Hawraa Kareem Department of Medical Physics - Hilla University College
Abstract :
This study aimed to investigate the immune responses of Penicillium digitatum antigens in rabbits. Twenty healthy rabbits were divided into 2 groups (15 for the test group) and (5 for the control group). The test animals were immunized with 500μg/ml of P. digitatum spores. The stimulated immune response was determined using Delayed type hypersensitivity-skin, Phagocyte activity (NBT), E-rosette, and leucocyte migration inhibitory factors (LIF) tests. The results of the delayed-type hypersensitivity test showed erythema in the skin thickness in immunized rabbits after 24, 48, and 72 hrs after injection of antigens, and necrosis after 72 hrs. The results showed increases in the percentage of phagocytic activity (74.333±1.329) compared to the control group (40.222±2.709), and a higher number of E-rosette (70.31±1.279) compared with control one (27.6±1.22). The results showed high significant LIF (55.66±2.11) in the treated group as well.
Keywords :
Delayed type hypersensitivity , skin test , NBT E , rosette test , LIF
Journal title :
Iranian Journal of Ichthyology
Journal title :
Iranian Journal of Ichthyology
