Isolation, identification, and antibiotic sensitivities of bacteria from sheep and human gallbladders

This investigation examined bacterial types in sheep gallbladders and human gallbladders with cholecystitis. Samples were collected to isolate and describe bacterial types, identify their relationship to cholecystitis, detect common germs in humans and sheep, and assess the sensitivity and resistance of bacterial isolates to antibiotics. The study found 56 sheep gallbladder isolates classified into nine types, including Staphylococcus aureus (12.8%), Streptococcus spp., Salmonella typhimurium and Pseudomonas aeruginosa (4.2%), E. coli (28.5%), Klebsiella spp., Listeria monocytogenes and Pasteurella spp. (5.7%), and Proteus spp. (8.5%). In contrast, in 78 human gallbladder isolates were found nine types, including S. aureus (18.3%), Listeria monocytogenes and Salmonella typhimurium (15%), E. coli (36.6%), Klebsiella spp. (8.3%), Proteus (11.6%), and Streptococcus spp. and Pasteurella spp. As a result, antibiotic isolates from sheep showed high sensitivity to antibiotics such as Ciprofloxacin, Rifampin, Ampicillin, Imipenem, and Gentamicin. In contrast, human isolates showed increased sensitivity to Imipenem, Ciprofloxacin, Vancomycin, Trimethoprim, and Rifampin, while the samples were highly resistant to Chloramphenicol, Erythromycin, Gentamicin, Ampicillin, and Cefotaxime. These findings suggest that antibiotic sensitivity and resistance are crucial factors in antibiotic resistance.