Title of article :
High Prevalence of Enterocytozoon bieneusi Genotype BEB6 in Wild Boars in Lorestan Province, Iran: Potential Source of Zoonotic Transmission
Author/Authors :
Javanmard ، Ehsan Department of Medical Parasitology and Mycology - School of Public Health, Student Scientific Research Center - Tehran University of Medical Sciences , Mohammad Rahimi ، Hanieh Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences , Taghipour ، Ali Department of Parasitology and Mycology - Zoonoses Research Center - Jahrom University of Medical Sciences , Nemati ، Sara Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences , Mohebali ، Mehdi Department of Medical Parasitology and Mycology - School of Public Health, Center for Research of Endemic Parasites of Iran (CREPI) - Tehran University of Medical Sciences , Rezaeian ، Mostafa Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences , Badrifar ، Ali Division of Avian Diseases - Islamic Azad University, Shoushtar Branch , Kazemirad ، Elham Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences , Mirjalali ، Hamed Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences
Abstract :
Background: We aimed to investigate the prevalence of Enterocytozoon bieneusi and the circulating genotypes in wild boar in the western regions of Iran. Methods: Fifty-two fecal samples were collected from wild boars in Lorestan province, Iran. After DNA extraction, the presence of E. bieneusi was evaluated by real-time PCR. A nested PCR targeting the internal transcribed region (ITS) was employed to characterize genotypes. The PCR products were sequenced, and the genetic diversity and relationships among the genotypes were identified using MEGA X and DnaSp (V5) software. Results: Sixteen (30%) samples were positive for E. bieneusi using real-time PCR, and 11 (21%) were positive for nested PCR, which was sequenced. All 11 positive samples were identified as the BEB6 genotype (Group 2). The haplotype diversity was noted to be 0.182, and the nucleotide diversity, calculated using DnaSp, was 0. 00085. Conclusion: The findings highlight the high prevalence of the genotype BEB6 in wild boars. The presence of this genotype suggests the circulation of E. bieneusi between domesticated animals and wild boars in Iran.
Keywords :
Enterocytozoon bieneusi , Wild boars , Iran , Molecular analysis
Journal title :
Iranian Journal of Parasitology (IJP)
Journal title :
Iranian Journal of Parasitology (IJP)
