Reproductive, hematological, and biochemical changes of wild female i Cyprinus carpio /i , subjected to induced reproduction by Ovulin and/or pituitary extract

The present study aimed to determine the effects of pituitary extract or Ovulin (containing s-GnRH and anti-dopamine) injection on reproductive parameters, hematological, and serum biochemical characteristics of the female Sazan carp, Cyprinus carpio. The female broodstocks were caught from the Caspian Sea and transferred to the Sijeval Center of Fish Propagation and Reconstruction of Genetic Reserves of Bony Fish in Bandar-e-Turkmen, Iran. After acclimation, the fish were divided into two experimental groups: one injected with pituitary extract (PT) and the other with Ovulin (OV). Blood samples were taken from the fish before the injections and 12 hours after, when egg samples were also collected. The results showed that OV treatment significantly increased number of egg, egg dimeter, fecundity, fertilization percentage, hatching percentage and survival percentage compared to PT treatment. Serum cortisol significantly increased in OV treatment after the injection. Serum glucose and erythrocyte count significantly increased after the injections, whereas serum cholesterol and sodium concentrations decreased. In conclusion, OV is beneficial as an alternative to PT extract to stimulate final maturation in female Sazan without any health problems.