Therapeutic efficacy of olive leave extract against Klebsiella pneumonia isolated from diabetic foot patients in vitro

Soft tissue infections are one of the most common among diabetes patients due to peripheral neuropathy involving the circulation of microvascular restricted phagocytic cell access to the infected area. The study aimed to investigate the bacteria in patients suffering from diabetic foot infections. The study included two main parts, the first was the bacterial diagnosis based on relied diagnostic procedures. The second part was to study the inhibitory effect of olive leaves on Klebsiella pneumonia and antimicrobial susceptibility. A total of 30 specimens were referred to Al-Hakim General Hospital in Al-Najaf province. The study was carried out at the bacterial laboratory in the Department of Medical Laboratory Techniques/ College of Medical and Health Technology/University of Al-kafeel in the province of Najaf. Gramme stain and a biochemical test were used for primary identification. Ultimately, the Vitek 2 system identification was completed. Out of the 30 clinical specimens, only 25 isolates were identified to be contaminated with Klebsiella pneumonia, according to the results. Alcoholic extracts of olive leaves were used by the Soxhlet extractor apparatus using ethanol and their inhibitory effect on the aforementioned bacteria was tested. The antimicrobial susceptibility test was examined by disk diffusion method, and the results have shown that there was significant inhibition of olive leaf extract on Klebsiella pneumonia with all concentration series.