Antibacterial and wound healing activities of biosynthesized AgNPs and ZnNPs from Sesamum indicum seeds extract against MDR Klebsiella variicola and Pantoea ananatis

Objective(s): Wound infection with multidrug resistant bacteria is a challenge of today’s medicine. Novel antibacterial agent such as nanoparticles are required to combat such threat. This study aims to find an alternative therapeutic agent to combat the drug resistant bacteria. Materials and Methods: During period (June to September 2022), 157 clinical samples were collected from different wound infections in different hospitals in Erbil city-Iraq. A total of 83 (52.86%) Gram negative bacteria were identified depending on 16S rRNA (PCR), sequencing and GenBank database. Silver, zinc and Ag–Zn composite nanoparticles from Sesamum indicum seeds were biosynthesized. Then, tested against Klebsiella variicola and Pantoea ananatis in vitro and in vivo using albino wistar rat model. Results: Findings showed that antibacterial effects with inhibition zone of each AgNPs and ZnNPs against K. variicola was 15 mm, while, for Ag-Zn composite nanoparticles was 12 mm. Otherwise, inhibition zone of the same NPs against P. ananatis was 14, 22 and 21 mm, respectively. The healing time varied among treatment groups. The healing time for wounds treated with NP-based creams were notably shorter compared to traditional treatments and non-treated group. The healing time of wounds infected with K. variicola treated by AgNPs, ZnNPs, Ag-Zn NPs, Vaseline, fusidin, mebo and non-treated groups were 13, 15, 14, 20, 16, 16 and 32 days, and for wounds infected with P. ananatis were 14,17, 14, 27, 28, 16 and 33 days, respectively. Conclusion: These results are promising in terms of nanoparticles’ potential ability to be used against pathogenic bacteria.